The most challenging problem is to maintain component consistency during component dynamic redistribution [述若干關(guān)鍵問題的解決方案和技術(shù)己在jzee應(yīng)用服務(wù)器onceasz中實(shí)現(xiàn)。
By using gfp - cam fusion protein , we have observed the detailed dynamic redistribution of cam in hela cells during cytokinesis . in tri - polar cell model , we have studied the relationship between the distribution pattern of cam and the formation of the cleavage furrow . furthermore , we have made the study on the regulation mechanism of cam during the whole process of cytokinesis by inhibiting its activity at different cytokinesis stages 我們采用綠色熒光蛋白( gfp )標(biāo)記技術(shù),重點(diǎn)觀察了胞質(zhì)分裂期hela細(xì)胞中g(shù)fp - cam的動(dòng)態(tài)分布;應(yīng)用三極細(xì)胞模型研究了cam的分布與分裂溝形成之間的關(guān)系;還通過在胞質(zhì)分裂不同階段抑制cam的活性研究了cam在整個(gè)胞質(zhì)分裂過程中的作用機(jī)制。